Composite

Part:BBa_K3102036

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)


T7-RBS-MTRC-Ter

This T7-RBS-MTRC-ter composite part is made of the Outer-membrane decaheme c-type cytochrome (Mtrc) which is involved in the metal reduction with MtrA and MtrB. All together they form a complex on the outer-membrane surface of the cell which transfers the electrons to the metal oxides. This part is crucial to catalyze the electron transfer from the outer membrane to the medium.

This construct is enriched with T7 promoter, RBS and terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 270


[edit]
Categories
Parameters
None